|
KMID : 0381120110330060673
|
|
Genes and Genomics
2011 Volume.33 No. 6 p.673 ~ p.678
|
|
|
Characterization of microRNAs regulating cyclooxygenase-2 gene expression
|
|
Yoon Se-Na
Choi Young-Chul
Lee Yu-Jin
Jin Min-Ho
Jeong Yong-Su
Yoon Jae-Seung
Baek Kwang-Hee
|
|
|
Abstract
|
|
|
|
Cyclooxygenase 2 (COX-2) is an enzyme for catalyzing the biosynthesis of prostanoids including prostaglandins and thromboxanes. In this study, miRNA-mediated regulation of COX-2 expression was investigated by employing 9 miRNAs (miR-26a, -101a, -143, -144, -145, -199a, -199a*, -542-3p, -543). Western blot analysis revealed that ectopic expression of miR-26a, -145, -199a, -542-3p, and -543 significantly reduced the level of COX-2 protein. The inhibitory effect of miR-143, -542-3p, and -543 on COX-2 expression appeared to be mediated via interaction with predicted binding sites in the 3′-untranslated region (3′-UTR) of COX-2 as revealed by luciferase reporter assay. In IL-1β-treated A549 cells, the production of prostaglandin E2 (PGE2) was significantly inhibited by miR-26a, -145, -199a, -542-3p, and -543. Together, our findings contribute to the understanding of post-transcriptional regulation of COX-2 and suggest an important role for miRNAs in COX-2 over-expression during inflammation and tumorigenesis.
|
|
|
KEYWORD
|
|
|
MicroRNA, Cyclooxygenase-2, PGE2, Post-transcriptional regulation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|